[Arisaema-l] Tissue culture of Arisaema from seed

[Dylan Condon]

Hi Aad,

I have found that a lot of bulb species are very difficult to initiate into culture using a twin scale segment or any part of the basal plate, and the only way to get them into culture clean is to use scape sections, immature flower buds, or any part of an immature inflorescence.

In the case of Hippeastrum, Murashige and Skoog would be the best basal media to use. Gamborgs is a completely different media, but M+S is the most widely used in tissue culture and you should be able to find this as complete media ready to use. BAP and IBA are plant growth regulators and won't generally be included in ready to use media, as the concentrations used will vary considerably depending on the plant being cultured. For the Hippeastrums you will need BAP and posssibly NAA and you should be able to buy these already in solution and you can just add them to the media at the rate you require.

This company in the USA should have the things that you need:
http://www.phytotechlab.com
and a bit of research on the web should dig up a few articles on the tissue culture of Hippeastrum and you can get an exact media from there.
Hope this helps and good luck!

Regards
Dylan



> On 3 Feb 2014, at 6:56 am, Aad van Beek <avbeek1 at hotmail.com> wrote:
> 
> Hi Dylan,
> 
> I'm a total newbie on tissue culture also. On facebook there was an interesting discussion on using tissue culture on inflorescence of hippeastrums and also on seeds from not fully ripen seedpods. I would like to try that but I'm not certain what medium to use. 
> 
> Found your Marashige and Skooge basal medium for instance sold by sigma-aldrich. But also enriched with Gamborg's vitamins and basal salt macronutrient. But could not find a Marashige medium with BAP and IBA. Does it mean the BAP and IBA ingredient have to be added or is there also a complete one in all solution including the agar on the market that would do for an initial try-out? 
> 
> Also any pointers on good articels, videos would be of great help.
> 
> Regards,
> Aad
> 
> From: cilladylan at iinet.net.au
> Date: Tue, 21 Jan 2014 20:35:36 +1100
> To: arisaema-l at science.uu.nl
> Subject: Re: [Arisaema-l] Tissue culture of Arisaema from seed
> 
> Hi Michael,
> 
> I run a commercial tissue culture lab here in Melbourne, Australia, and have done some work on Arisaema in the past, both for my own personal interest and for a nursery interested in growing them on from culture. I initiated material from both seed and the shoot tips from small tubers, and actually had more success from the latter, as far as active growth and decent multiplication rates are concerned.
> I originally thought I would try and multiply them as small tubers in culture and try to get them to produce offsets, but I found it much more productive to culture them as a cluster of shoots and then divide them into individual plantlets and get them to produce a small tuber at the base of the shoot once on rooting media.
> The species I worked on were Serratum, Consanguineum, Candidissimum, and Erubescens.
> All behaved in a fairly similar way in culture, and all grew on Murashige and Skoog with BAP and IBA in varying concentrations, so finding an appropriate media shouldn't be too much of a challenge.
> Good luck with your project and if I can be of any assistance or you would like some more detailed information then just let me know.
> 
> Regards
> Dylan
> 
> 
> 
> 
> On 21 Jan 2014, at 2:47 pm, Michael Homick <michaelhomick at gmail.com> wrote:
> 
> 
> 
> 
> I have set up a "tissue culture room" attached to my home with a commercial laminar flow hood, growing shelves etc. The hood cost me $50 on ebay. I will be experimenting on Lilium seed, bulbs and ovule culture over the next year but would also like to look into the possibility of Arisaema culture from seed. There is all kinds of articles on culturing lilies, but I am unable to find any protocol on Arisaema, other than a mention by Tony Advent in one of his blogs about Arisaema serratum var. serratum 'Uulong Treasure' being cultured, However this would not have been from seed.
> 
> Once/if I develop the proper procedure for Arisaema seed sterilization, I hope to compare various media
> 
> i.e. Murashige and Skoog Media and various Terrestrial Orchid Media   as a starting point.
> 
>  
> 
> If anyone from this group has any comments or suggestions, please feel free to get in touch. I hope to experiment on some of the more common species from the seed exchange this spring. If successful in germinating seed in vitro and finding out if it is possible to multiply the resulting seedlings, there is a chance of being successful with rarer material in the future. 
> 
>  
> 
> If members of the group feel this journey is relevant to the Discussion List, I will keep them updated on any progress, otherwise if people just wish to correspond privately do so as well.
> 
>  
> 
> All and any comments, suggestions links to relevant articles very appreciated.
> 
>  
> 
> All the best,
> 
> Michael Homick
> 
> michaelhomick at gmail.com
> 
> Stevinson, CA. USA
> 
> 
> 
> 
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