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Fri Jun 9 19:03:39 CEST 2006

hardy  Aroids)" <ARISAEMA-L at NIC.SURFNET.NL> Aroids)" <ARISAEMA-L at NIC.SURFNET.NL>
Sender: "Arisaema Enthusiast Group (AEG) Discussion List (and other=
From: Stacy Holtzman <sholtzma at INDIANA.EDU>
Subject: Re: Arisaema tissue culture
In-Reply-To: <448992FA.6010500 at plantdelights.com>
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Then it appears that expanding the gene pool would be likely to give the
most interesting results - by getting the inherited patterning enhanced o=r
altered by artificial selection of the most interesting/different pattern=s.
I wonder if anybody has explored the nature of the genetics of that
patterning, and how much "natural" variation there is to this patterning?=We
could do the equivalent of eHarmony and post our silver centers for matin=g.

Stacy Holtzman

Bloomington Drosophila Stock Center

Biology Dept., Jordan Hall A512

1001 E. Third St.

Bloomington, IN  47405-3700

Phone: 812-855-5782

Fax: 812-855-2577

_____

From: Arisaema Enthusiast Group (AEG) Discussion List (and other hardy
Aroids) [mailto:ARISAEMA-L at NIC.SURFNET.NL] On Behalf Of Tony Avent
Sent: Friday, June 09, 2006 11:26 AM
To: ARISAEMA-L at NIC.SURFNET.NL
Subject: Re: Arisaema tissue culture

Henry:

We haven't got any variegated arisaema yet...just hoping to do so from th=e
tc process.  If the variegation is a stable chimera, then tc usually has =no
problems producing true to type offspring.  If the variegation is sectore=d,
then reproduction through tissue culture is difficult.  The silver patter=ns
are not really a true variegation and therefore reproduce fine via tc.

Tony Avent
Plant Delights Nursery @
Juniper Level Botanic Garden
9241 Sauls Road
Raleigh, North Carolina  27603  USA
Minimum Winter Temps 0-5 F
Maximum Summer Temps 95-105F
USDA Hardiness Zone 7b
email tony at plantdelights.com
website  http://www.plantdelights.com
phone 919 772-4794
fax  919 772-4752
"I consider every plant hardy until I have killed it myself...at least th=ree
times" - Avent

Henry wrote:

Tony,

I understand that with some variegated plants, tissue culture will not
capture the variegation.

If the starting tissue is the green part of the leaf you will get a green
plant, if the sample is in the silver part, you get a silver plant.

Do the variegated arisaema follow this rule?

--Henry Fieldseth

Minneapolis, Minnesota, USA, zone 4

PS

The A. sikokianum was a quick sell-out at the rare plants table at our
spring sale.

http://fsmn.org/events_plant.html

Tony Avent <mailto:tony at PLANTDELIGHTS.COM> <tony at PLANTDELIGHTS.COM> wrot=e:

Ellen:

You are correct in that some species don't need tc unless you are looking
for really large numbers.  After five years of tc, we have not seen a sin=gle
mutant yet.  I'm still looking for the white edged forms, but no luck.
Those with silver patterns do not show up as patterned until they mature =and
have gone through two dormancies.

_____

Sneak preview the all-new
<http://us.rd.yahoo.com/evt=40762/*http:/www.yahoo.com/preview> Yahoo.=com.
It's not radically different. Just radically better.

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<div class=Section1>

<sp=an =
style='font-size:
10.0pt;font-family:Arial;color:navy'>Then it appears that expanding the =
gene
pool would be likely to give the most interesting results &#8211; by =
getting
the inherited patterning enhanced or altered by artificial selection of =
the
most interesting/different patterns.&nbsp; I wonder if anybody has =
explored the
nature of the genetics of that patterning, and how much =
&#8220;natural&#8221;
variation there is to this patterning? We could do the equivalent of =
eHarmony
and post our silver centers for mating.<o:p></o:p>

<p class=MsoNormal><font size=2 color=navy face=Arial><sp=an =
style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p>&nbsp;</o:p></span></font></p>

<div>

<p class=MsoAutoSig><font size=3 color=navy face="Times N=ew =
Roman"><span
style='font-size:12.0pt;color:navy'>Stacy =
Holtzman<o:p></o:p></span></font></p>

<p class=MsoAutoSig><st1:place w:st="on"><st1:PlaceName =
w:st="on"><font size=3
color=navy face="Times New Roman"><span =
style='font-size:12.0pt;color:navy'>Bloomington</span></font></st1:Pl=ac=
eName><font
color=navy><span style='color:navy'> <st1:PlaceName =
w:st="on">Drosophila</st1:PlaceName>
<st1:PlaceName w:st="on">Stock</st1:PlaceName> <st1:PlaceType =
w:st="on">Center</st1:PlaceType></span></font></st1:place><font
color=navy><span style='color:navy'><o:p></o:p></span></font></p>

<p class=MsoAutoSig><font size=3 color=navy face="Times N=ew =
Roman"><span
style='font-size:12.0pt;color:navy'>Biology Dept., Jordan Hall =
A512<o:p></o:p></span></font></p>

<st1:Street w:st="on"><st1:address =
w:st="on">1001
E. Third St</st1:address></st1:Street>.<o:p></o:p>

<p class=MsoAutoSig><st1:place w:st="on"><st1:City w:st="on">=<font =
size=3
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style='font-size:12.0pt;color:navy'>Bloomington</span></font></st1:Ci=ty=
><font
color=navy><span style='color:navy'>, <st1:State =
w:st="on">IN</st1:State>&nbsp;
<st1:PostalCode =
w:st="on">47405-3700</st1:PostalCode></span></font></st1:place><font
color=navy><span style='color:navy'><o:p></o:p></span></font></p>

<p class=MsoAutoSig><font size=3 color=navy face="Times N=ew =
Roman"><span
style='font-size:12.0pt;color:navy'>Phone: =
812-855-5782<o:p></o:p></span></font></p>

<p class=MsoAutoSig><font size=3 color=navy face="Times N=ew =
Roman"><span
style='font-size:12.0pt;color:navy'>Fax: =
812-855-2577<o:p></o:p></span></font></p>

</div>

<p class=MsoNormal><font size=2 color=navy face=Arial><sp=an =
style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p>&nbsp;</o:p></span></font></p>

<div>

<div class=MsoNormal align=center style='text-align:center'><=font =
size=3
color=black face="Times New Roman"><span =
style='font-size:12.0pt;color:windowtext'>

<hr size=2 width="100%" align=center tabindex=-1>

</span></font></div>

<p class=MsoNormal><b><font size=2 color=black face=Tahom=a><span
style='font-size:10.0pt;font-family:Tahoma;color:windowtext;font-weig=ht=
:bold'>From:</span></font></b><font
size=2 color=black face=Tahoma><span =
style='font-size:10.0pt;font-family:Tahoma;
color:windowtext'> Arisaema Enthusiast Group (AEG) Discussion List (and =
other
hardy Aroids) [mailto:ARISAEMA-L at NIC.SURFNET.NL] <b><span =
style='font-weight:
bold'>On Behalf Of </span></b>Tony Avent<br>
<b><span style='font-weight:bold'>Sent:</span></b> Friday, June 09, =
2006 11:26
AM<br>
<b><span style='font-weight:bold'>To:</span></b> =
ARISAEMA-L at NIC.SURFNET.NL<br>
<b><span style='font-weight:bold'>Subject:</span></b> Re: Arisaema =
tissue
culture</span></font><font color=black><span =
style='color:windowtext'><o:p></o:p></span></font></p>

</div>

<p class=MsoNormal><font size=3 color=black face="Times N=ew =
Roman"><span
style='font-size:12.0pt'><o:p>&nbsp;</o:p></span></font></p>

<p class=MsoNormal><font size=3 color=black face="Times N=ew =
Roman"><span
style='font-size:12.0pt'>Henry:<br>
<br>
We haven't got any variegated arisaema yet...just hoping to do so from =
the tc
process.&nbsp; If the variegation is a stable chimera, then tc usually =
has no
problems producing true to type offspring.&nbsp; If the variegation is
sectored, then reproduction through tissue culture is difficult.&nbsp; =
The
silver patterns are not really a true variegation and therefore =
reproduce fine
via tc.<br>
<br>
<o:p></o:p></span></font></p>

<pre><font size=2 color=black face="Courier New"><span =
style='font-size:10.0pt'>Tony =
Avent<o:p></o:p></span></font></pre><pre><font
size=2 color=black face="Courier New"><span =
style='font-size:10.0pt'>Plant Delights Nursery =
@<o:p></o:p></span></font></pre><pre><st1:place
w:st="on"><st1:PlaceName w:st="on"><font size=2 color=bla=ck =
face="Courier New"><span
style='font-size:10.0pt'>Juniper</span></font></st1:PlaceName> =
<st1:PlaceName
w:st="on">Level</st1:PlaceName> <st1:PlaceType w:st="on">Botanic==
Garden</st1:PlaceType></st1:place><o:p></o:p></pre><pre><st1:Street
w:st="on"><st1:address w:st="on"><font size=2 color=black==
face="Courier New"><span
style='font-size:10.0pt'>9241 Sauls =
Road</span></font></st1:address></st1:Street><o:p></o:p></pre><pre><st1:p==
lace
w:st="on"><st1:City w:st="on"><font size=2 color=black =
face="Courier New"><span
style='font-size:10.0pt'>Raleigh</span></font></st1:City>, =
<st1:State w:st="on">North Carolina</st1:State>&nbsp; <st1:PostalCode
w:st="on">27603</st1:PostalCode>&nbsp; <st1:country-region =
w:st="on">USA</st1:country-region></st1:place><o:p></o:p></pre><pre><=fo=
nt
size=2 color=black face="Courier New"><span =
style='font-size:10.0pt'>Minimum Winter Temps 0-5 =
F<o:p></o:p></span></font></pre><pre><font
size=2 color=black face="Courier New"><span =
style='font-size:10.0pt'>Maximum Summer Temps =
95-105F<o:p></o:p></span></font></pre><pre><font
size=2 color=black face="Courier New"><span =
style='font-size:10.0pt'>USDA Hardiness Zone =
7b<o:p></o:p></span></font></pre><pre><font
size=2 color=black face="Courier New"><span =
style='font-size:10.0pt'>email <a
href="mailto:tony at plantdelights.com">tony at plantdelights.com</a><o:p><=/o=
:p></span></font></pre><pre><font
size=2 color=black face="Courier New"><span =
style='font-size:10.0pt'>website&nbsp; <a
href="http://www.plantdelights.com">http://www.plantdelights.com</a><=o:=
p></o:p></span></font></pre><pre><font
size=2 color=black face="Courier New"><span =
style='font-size:10.0pt'>phone 919 =
772-4794<o:p></o:p></span></font></pre><pre><font
size=2 color=black face="Courier New"><span =
style='font-size:10.0pt'>fax&nbsp; 919 =
772-4752<o:p></o:p></span></font></pre><pre><font
size=2 color=black face="Courier New"><span =
style='font-size:10.0pt'>&quot;I consider every plant hardy until I =
have killed it myself...at least three times&quot; - =
Avent<o:p></o:p></span></font></pre>

<p class=MsoNormal><font size=3 color=black face="Times N=ew =
Roman"><span
style='font-size:12.0pt'><br>
<br>
Henry wrote: <o:p></o:p></span></font></p>

<div>

<p class=MsoNormal><font size=3 color=black face="Times N=ew =
Roman"><span
style='font-size:12.0pt'>Tony,<o:p></o:p></span></font></p>

</div>

<div>

<p class=MsoNormal><font size=3 color=black face="Times N=ew =
Roman"><span
style='font-size:12.0pt'>&nbsp;<o:p></o:p></span></font></p>

</div>

<div>

<p class=MsoNormal><font size=3 color=black face="Times N=ew =
Roman"><span
style='font-size:12.0pt'>I understand that with some variegated =
plants, tissue
culture will not capture the variegation.<o:p></o:p></span></font></p>

</div>

<div>

<p class=MsoNormal><font size=3 color=black face="Times N=ew =
Roman"><span
style='font-size:12.0pt'>&nbsp;<o:p></o:p></span></font></p>

</div>

<div>

<p class=MsoNormal><font size=3 color=black face="Times N=ew =
Roman"><span
style='font-size:12.0pt'>If the starting tissue is the green part of ==
the leaf
you will get a green plant, if the&nbsp;sample is in the silver part, =
you get a
silver plant.<o:p></o:p></span></font></p>

</div>

<div>

<p class=MsoNormal><font size=3 color=black face="Times N=ew =
Roman"><span
style='font-size:12.0pt'>&nbsp;<o:p></o:p></span></font></p>

</div>

<div>

<p class=MsoNormal><font size=3 color=black face="Times N=ew =
Roman"><span
style='font-size:12.0pt'>Do the variegated arisaema follow this =
rule?<o:p></o:p></span></font></p>

</div>

<div>

<p class=MsoNormal><font size=3 color=black face="Times N=ew =
Roman"><span
style='font-size:12.0pt'>&nbsp;<o:p></o:p></span></font></p>

</div>

<div>

<p class=MsoNormal><font size=3 color=black face="Times N=ew =
Roman"><span
style='font-size:12.0pt'>--Henry =
Fieldseth<o:p></o:p></span></font></p>

</div>

<div>

<p class=MsoNormal><st1:place w:st="on"><st1:City w:st="on"><=font =
size=3
color=black face="Times New Roman"><span =
style='font-size:12.0pt'>Minneapolis</span></font></st1:City>,
<st1:State w:st="on">Minnesota</st1:State>, <st1:country-region =
w:st="on">USA</st1:country-region></st1:place>,
zone 4<o:p></o:p></p>

</div>

<div>

<p class=MsoNormal><font size=3 color=black face="Times N=ew =
Roman"><span
style='font-size:12.0pt'>&nbsp;<o:p></o:p></span></font></p>

</div>

<div>

<p class=MsoNormal><font size=3 color=black face="Times N=ew =
Roman"><span
style='font-size:12.0pt'>PS <o:p></o:p></span></font></p>

</div>

<div>

The A. sikokianum was a quick =
sell-out&nbsp;at the
rare plants table at our spring sale.<o:p></o:p>

</div>

<div>

<p class=MsoNormal><font size=3 color=black face="Times N=ew =
Roman"><span
style='font-size:12.0pt'><a =
href="http://fsmn.org/events_plant.html">http://fsmn.org/events_plant=.h=
tml</a><o:p></o:p></span></font></p>

</div>

<div>

<p class=MsoNormal><font size=3 color=black face="Times N=ew =
Roman"><span
style='font-size:12.0pt'><br>
<br>
<b><i><span style='font-weight:bold;font-style:italic'>Tony Avent <a
href="mailto:tony at PLANTDELIGHTS.COM">&lt;tony at PLANTDELIGHTS.COM&gt;</=a>=
</span></i></b>
wrote:<o:p></o:p></span></font></p>

</div>

<blockquote style='border:none;border-left:solid #1010FF =
1.5pt;padding:0in 0in 0in 4.0pt;
margin-left:3.75pt;margin-top:5.0pt;margin-bottom:5.0pt'>

<p class=MsoNormal><font size=3 color=black face="Times N=ew =
Roman"><span
style='font-size:12.0pt'>Ellen:<br>
<br>
You are correct in that some species don't need tc unless you are =
looking for
really large numbers.&nbsp; After five years of tc, we have not seen a =
single
mutant yet.&nbsp; I'm still looking for the white edged forms, but no
luck.&nbsp; Those with silver patterns do not show up as patterned until ==
they
mature and have gone through two =
dormancies.<o:p></o:p></span></font></p>

</blockquote>

<div class=MsoNormal align=center style='text-align:center'><=font =
size=3
color=black face="Times New Roman"><span style='font-size:12.=0pt'>

<hr size=1 width="100%" align=center>

</span></font></div>

Sneak preview the <a
href="http://us.rd.yahoo.com/evt=40762/*http:/www.yahoo.com/previ=ew">=
all-new
Yahoo.com</a>. It's not radically different. Just radically better. =
<o:p></o:p>

</div>

</body>

</html>

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