[Arisaema-l] Tissue culture of Arisaema from seed

[don]

Michael,Although I have no expertise in this area, I would be very interested in any and all results and/or failures in this adventure.All the best,Don.

Date: Mon, 20 Jan 2014 19:47:02 -0800
From: michaelhomick at gmail.com
To: arisaema-l at science.uu.nl
Subject: [Arisaema-l] Tissue culture of Arisaema from seed




I have set up a "tissue culture room" attached to my
home with a commercial laminar flow hood, growing shelves etc. The hood cost me
$50 on ebay. I will be experimenting on Lilium seed, bulbs and ovule culture
over the next year but would also like to look into the possibility of Arisaema
culture from seed. There is all kinds of articles on culturing lilies, but I am
unable to find any protocol on Arisaema, other than a mention by Tony Advent in
one of his blogs about Arisaema serratum var. serratum 'Uulong Treasure' being
cultured, However this would not have been from seed.

Once/if I develop the proper procedure for Arisaema seed
sterilization, I hope to compare various media

i.e. Murashige and Skoog Media and various Terrestrial
Orchid Media   as a starting point.

 

If anyone from this group has any comments or suggestions,
please feel free to get in touch. I hope to experiment on some of the more
common species from the seed exchange this spring. If successful in germinating
seed in vitro and finding out if it is possible to multiply the resulting
seedlings, there is a chance of being successful with rarer material in the
future. 

 

If members of the group feel this journey is relevant to the
Discussion List, I will keep them updated on any progress, otherwise if people
just wish to correspond privately do so as well.

 

All and any comments, suggestions links to relevant articles very
appreciated.

 

All the best,

Michael Homick

michaelhomick at gmail.com

Stevinson, CA. USA





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